Revisión sistemática de los efectos de microvibración de baja magnitud alta frecuencia en osteocitos cultivados.

Abstract

Introduction: The bone reservoir of minerals and organic molecules is a dynamic tissue that detects and adapts to the mechanical loads of the organs and tissues of the body, which maintains the bone structure of the skeleton during growth and throughout the life of the human being. bone cells are sensitive to mechanical loads and microvibrations received by the skeleton. Objective: The purpose of this study was to carry out a systematic review about the effects exerted by high frequency-low intensity micro-vibration in osteocytes cultured in vitro on the synthesis of soluble factors, to understand if micro vibration has an influence on the acceleration of tooth movement. Material and methods: A search was carried out for review articles on osteocytes and other bone cells in vitro, establishing the PICO strategy, using keywords such as: «osteocytes», «micro-vibration», «remodeling», «osteoclastogenesis», «cytokines», «osteoblasts», was structured using PRISMA and final data capture using the Jadad and Cochrane method, evaluating the risk of bias of each of the articles. Eleven articles with high methodological quality were included. Results: Most of the in vitro experiments showed that micro-vibration had a statistically significant increase in the proliferation and differentiation of mesenchymal stem cells (MSC), in osteoblasts (MC3T3-E1) in the protein expression to induce osteogenesis, osteocytes (MLO-Y4) upregulated the expression of osteoprotegerin (OPG), prostaglandin (PGE2) and nitrous oxide (NO) by altering and regulating soluble factors such as cytokines, growth factors and chemokines of the other cells, in addition to showing a decrease in osteoclast activity RAW246.7) in bone resorption. Conclusion: Micro-vibration induces bone remodeling, osteocytes are sensitive to mechanical stimuli and produce soluble factors to induce bone remodeling, therefore microvibration is used as an innovative and promising non-invasive and non-pharmacological therapy, in stimulating bone formation on the bone surface. (English) [ABSTRACT FROM AUTHOR]