The inhibitory effect of garlic and onion root exudates on escherichia coli from urinary tract infection and molecular detection of hlya virulence gene

Abstract

162 urine samples were collected from UTIs during October to December 2017 at Saladin General Hospital. E. coli was 38.9% of the collected samples. Women are more vulnerable to infection. The resistance percentage to Chloramphenicol, Ofloxacin, Ceftriaxone and Imipenem were 17% to 100%. The root exudates were prepared by using hydroponics with different periods: (7, 10 and 14 days). Onion (Allium cepa L.) root exudates have not any effect on the E. coli through the periods while root exudates of garlic (Allium sativum L.) gave the highest inhibitory effect at the period of 10 and 14 days. The virulence factors of E. coli were screened for the presence of hlyA through PCR and showed that all the resistant isolates could produce beta-hemolysis.