Physiological quality of stored dipteryx alata vogel seeds:tetrazolium test and radiographic imaging standardization

Abstract

The baru (Dipteryx alata Vogel), is fruit tree displaying economic, environmental, industrial and food importance. The objective of this study was to adapt to the tetrazolium test methodology to baru seeds and evaluate the physiological quality of seeds stored for 12 months at 10 and 20 ºC, using X-ray images as a toll for detection damage throughout the storage period while also carrying out germination, emergence and electrical conductivity tests. Baru seeds remained viable for 12 months of storage, albeit with a small decrease in vigor after nine months. The applied tetrazolium test methodology was efficient in determining internal seed damage. The pre-soak time for the tetrazolium test and solution concentration was 24 hours and 0.05%. No changes in seed morphology and radiographic images were observed during the storage periods, regardless of the assessed temperature. The X-ray test enabled a precise visualization of internal baru seed structures.