Isolation and identification probiotic candidates bacteria from Crossbreed Fries Holland (CFH) colostrum that grown on nutrient agar with olive oil and casein enrichment

Abstract

Aims of the research were to isolate the bovine colostrum indigenous bacteria that potent as probiotics with Nutrient Agar (NA) as a growth media. Selected isolate expected to develop as anti-pathogen in food spoilage and digestion. Material used including bovine colostrum taking from lactating Crossbreed Fries Holland (CFH) 1-3 days after partus which done compositely. Crossbreed Fries Holland colostrum everyday was taken 250 mL at the same time in the morning and afernoon and then stored at the refrigerator. Research done with qualitative method which analyzes descriptively through isolation step cover: instrument sterilization, dilution, morphology observation and pure culture production, then continued with identification step (gram staining, catalase test, oxidase test, low pH, high bile salt and API Test). Result showed that isolation with NA with olive oil addition obtained 10 bacteria (5 Bacillus genus, 3 Micrococcus genus and Staphylococcus genus). While at the NA with 1% casein addition obtained 12 pure isolate including: 2 Staphylococcus genus, 5 Bacillus genus, 2 Micrococcus genus and each Streptococcus, Enterobacter and Veillonella was found 1. Catalase and oxidase test resulting in two groups that cover Bacillus and Coccus group. Twenty isolate identified as gram positive bacteria, while two genus such as Enterobacter and Veillonella was identified as gram negative bacteria. Gram positive bacteria tested to determine the species using API test and resulting two dominant bacteria species with code Ic8 for Bacillus coagulans species with 93.7% similarity, Ic12 for Aneurinibacillus aneurinilyticus with 99.1% similarity.