Biodegradation of aliphatic waste components of oil sludge used micro symbiont of Sponge Niphates sp.

Abstract

Investigation has been carried out using a sponge Niphates sp. as biodegradation agent of the aliphatic hydrocarbon component of crude oil sludge. The experiment was carried out by colonizing and making suspension of Bacillus pumilus strains GLB197 and Bacillus cohnii strains of DSM 6307, isolated from sponge Niphates sp. Both types of micro symbiont suspension were mixed with sludge waste for 30 days. There are five biodegradation parameters observed, carried out every five days of incubation. Parameters that observed during incubation were pH, gas bubbles and fermentation odor. Meanwhile, the optical density of the media was measured using spectrometer-D20+, Aliphatic components degradation was measured using GC-MS. Observation of biodegradation parameters showed there was a change in pH from 7 to 6, formed gas bubbles, resulting in fermentation odors and showed an increased optical density by an average of 35%. These four parameters occur during the interaction of 10-20 days of interaction in the media. There were 20 types of aliphatic components identified in the crude oil sludge waste. Analysis of four aliphatic components, namely Tridecane, Octadecane, Tricosane, and Nonacosane showed a decrease in the average concentration of 48.11 %.