Agrobacterium rhizogenes – Mediated hairy root induction in garlic

Abstract

Three factors including Agrobacterium rhizogenes strains (A4, A7 and ATCC15834), genotypes (Gorgan and Ramhormoz) and explant types (stem disc meristem, shoot apical meristem and root apical meristem) were conducted to study the effects on hairy root induction of garlic. Our results showed that A. rhizogenes strains and explants types had significant effect on root induction frequency (RIF), while the genotypes hadn’t effect on the RIF. The frequency of root induction was up to 23.3%. Some the combination of three factors could induce hairy roots.The results of PCR analysis showed that expected fragments with length of 780bp corresponding to the rolB gene was amplified from hairy root cultures. To our knowledge, this study was the first comprehensive evaluation of garlic hairy root induction.