Seasonal trends of light-saturated net photosynthesis and stomatal conductance of loblolly pine trees grown in contrasting environments of nutrition, water and carbon dioxide

Abstract

Repeated measures analysis was used to evaluate the effect of long-term CO2 enhancement on seasonal trends of light-saturated rates of net photosynthesis (A(sat)) and stomatal conductance to water vapour (g(sat)) of 9-year-old loblolly pine (Pinus taeda L.) trees grown in a 2 x 2 factorial experimental design of nutrition and water. A significant interaction effect of CO2 and nutrition on mean A(sat) was observed for juvenile foliage. Also, juvenile foliage exposed to +350 μmol mol-1 CO2 had a higher rate of increase of A(sat) between late summer and early autumn. This would lead to a greater potential for recharging carbohydrate reserves for winter. Mature foliage was affected by CO2, water and nutrient treatments in two ways. First, A(sat) was significantly increased as a result of elevated CO2 in January, a period when stomatal conductance was only 47% of the maximum observed rate. Secondly, the rate of increase of A(sat) from winter to early spring was accelerated as a result of both nutrient + water and + 350 μmol mol-1 CO2 treatments. This accelerated response resulted in a greater potential for photosynthate production during the period when growth initiation occurred. Nutrient, water or carbon dioxide treatments did not significantly alter trends in g(sat) for mature or juvenile foliage. A significant nutrition x CO2 interaction was observed for the mature foliage, suggesting that g(sat) increased with increasing CO2 and nutrition. These results may have important consequences for the determination of the water use efficiency of loblolly pine. In spite of low g(sat) in the winter to early spring period, there was a substantial gain in A(sat) attributable to elevated CO2 concentrations.