Antimicrobial, antioxidant and cytotoxic activities of Glochidion multiloculare (Roxb. ex Willd.) Müll. Arg. (Euphorbiaceae)

Abstract

The methanol extract (ME) of the powdered bark of Glochidion multiloculare and its six vacuum liquid chromatographic (VLC) fractions (Fa-f) were investigated for antimicrobial, cytotoxic and antioxidant activities. Only fractions Fc and Fd showed mild antimicrobial activity. Significant free radical (DPPH) scavenging activity was found in Ff (IC50 value = 9.27±0.117 μg/ml). The total phenolic content was measured involving Folin-Ciocalteu reagent and it was the highest in fraction Fe (187.00±1.74 mg of GAE/gm of sample). Cytotoxicity (LC50) by brine shrimp lethality bioassay was found to be significant for Fb (0.023±0.001 μg/ml), Fc (0.3±0.01 μg/ml) and Fd (0.117±0.0015 μg/ml).