Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Abstract

The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The majorcomponent in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reducedclotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatestinhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases inclotting time were also observed, with values considered significant between 10-27 seconds. Lysis values50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysisinduced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in thecomet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wideapplication potential considering the pharmacological properties observed in this study