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Protoplast regeneration in Clostridium tertium: Isolation of derivatives with high-frequency regeneration

Applied and Environmental Microbiology (1984) 48(6) 1246-1247
DOI: 10.1128/aem.48.6.1246-1247.1984
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Abstract

A procedure was developed for the formation and regeneration of protoplasts in Clostridium tertium. Although in the parent strain most of the protoplasts gave rise to L-form colonies, derivatives could be isolated which exhibited high regeneration frequencies.

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Knowlton, S., Ferchak, J. D., & Alexander, J. K. (1984). Protoplast regeneration in Clostridium tertium: Isolation of derivatives with high-frequency regeneration. Applied and Environmental Microbiology, 48(6), 1246–1247. https://doi.org/10.1128/aem.48.6.1246-1247.1984

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