Bioorganically doped sol-gel materials containing amyloglucosidase activity

Abstract

Amyloglucosidase (AMG) from Aspergillus niger was encapsulated in various matrices derived from tetraethoxysilane, methyltriethoxysilane, phenyltriethoxysilane and vinyltriacetoxysilane by different methods of immobilization. The immobilized enzyme was prepared by entrapment in two steps, in one-step and entrapment/deposition, respectively. The activities of the immobilized AMG were assayed and compared with that of the native enzyme. The effects of the organosilaneprecursors and their molar ratios, the immobilization method, the inorganic support (white ceramic, red ceramic, purolite, alumina, TiO2, celite, zeolite) and enzyme loading upon the immobilized enzyme activity were tested. The efficiency of the sol-gel biocomposites can be improved through combination of the fundamental immobilization techniques and selection of the precursors.Amiloglukozidaza (AMG) iz gljive Aspergillus niger, je inkapsulirana u kapsulu sa razlicitim matriksima koji su dobijeni od tetraetoksisilana, metiltrietoksisilana, feniltrietoksisilana i viniltriacetoksilana, razlicitim metodama imobilizacije. Imobilizacija enzima je postignuta u dva koraka, u jednoj fazi i inkapsuliranjem/depozicijom, redom. Aktivnosti imobilisane AMG su ispitane i poredjene sa nevezanim enzimom. Ispitivani su uticaj prekursora na bazi organosilana i njihov molarni odnos, metoda imobilizacije, neorganski nosac (bela keramika, crvena keramika, alumina, TiO2, zeoliti, celit, purolit) i aktivnost imobilisanog enzima. Efikasnost sol-gel biokompozita moze se poboljsati kombinacijom osnovnih tehnika imobilizacije i izborom prekursora.