Latency of auditory evoked potential monitoring the effects of general anesthetics on nerve fibers and synapses

Abstract

Auditory evoked potential (AEP) is an effective index for the effects of general anesthetics. However, it's unknown if AEP can differentiate the effects of general anesthetics on nerve fibers and synapses. Presently, we investigated AEP latency and amplitude changes to different acoustic intensities during pentobarbital anesthesia. Latency more regularly changed than amplitude during anesthesia. AEP Latency monotonically decreased with acoustic intensity increase (i.e., latency-intensity curve) and could be fitted to an exponential decay equation, which showed two components, the theoretical minimum latency and stimulus-dependent delay. From the latency-intensity curves, the changes of these two components (ΔL and ΔI) were extracted during anesthesia. ΔL and ΔI monitored the effect of pentobarbital on nerve fibers and synapses. Pentobarbital can induce anesthesia, and two side effects, hypoxemia and hypothermia. The hypoxemia was not related with ΔL and ΔI. However, ΔL was changed by the hypothermia, whereas ΔI was changed by the hypothermia and anesthesia. Therefore, we conclude that, AEP latency is superior to amplitude for the effects of general anesthetics, ΔL monitors the effect of hypothermia on nerve fibers, and ΔI monitors a combined effect of anesthesia and hypothermia on synapses. When eliminating the temperature factor, ΔI monitors the anesthesia effect on synapses.