A High Potential of Kaempferia parviflora Cell Culture for Phenolics and Flavonoids Production

Abstract

Background and Objective: Bioactive compound production has been facing many challenges including a long cultivation time, the instability and the impurity of the products from natural plants. This study aimed to produce bioactive compounds from K. parviflora by using plant cell culture system and determine their antioxidant potential. Materials and Methods: Callus was induced from different organs of the plant including the shoots, rhizomes and roots using Murashige and Skoog (MS) medium supplemented with various Plant Growth Regulators (PGRs). Fast-growing friable calli from all explants were selected and used to establish cell suspension cultures. The biomass production, the accumulation of total phenolics, total flavonoids and the antioxidant activity of the cell culture extracts were determined and compared with those from intact rhizomes of K. parviflora. Results: All cell suspension cultures of K. parviflora exhibited high biomass yields. Furthermore, the productivities of the phenolics and flavonoids from K. parviflora cell cultures were also significantly higher than those from the intact rhizomes. The antioxidant capacity of the cell culture extracts was found to be comparable with that detected in the intact rhizomes of this plant. Conclusion: Based on the high biomass yield, high bioactive compounds productivity and antioxidant capacity, K. parviflora cell culture system displayed great potential for commercial-scale production. Further investigations on the multiple biological properties of the bioactive compounds produced by K. parviflora cell suspension cultures as well as the cytotoxicity test will be explored