The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice ( Mus musculus )

Abstract

Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).