Medical bioproducts collagen quantification by hydroxyproline determination

Abstract

The paper presents a method of quantifying collagen from medical collagen-based bioproducts obtained in the Collagen Research Department of INCDTP - Division ICPI by determining hydroxyproline. Collagen differs from usual proteins in that it contains a higher concentration of certain amino acids. Almost a third of collagen is made up of glycine, the smallest amino acid, and another third is made up of proline and hydroxyproline, the active form of proline. As hydroxyproline has been found in very few proteins other than collagen, hydroxyproline determination is used as a marker to quantify collagen levels from various medical products. Collagen is quantified by multiplying hydroxyproline content by the 6.6 factor. The proposed method was validated to establish performance parameters and to check accordance with the set goal, by determining: limit of detection, limit of quantification, selectivity, sensitivity, robustness, accuracy and reliability of the method.