Morphometry of retinal vasculature in Antarctic fishes is dependent upon the level of hemoglobin in circulation

Abstract

We quantitatively assessed ocular vascular patterns of six Antarctic notothenioid fishes that vary in their expression of the circulating oxygen-binding protein, hemoglobin (Hb). Digital image analyses revealed marked differences in vessel morphometries among notothenioid species. Hemoglobinless (-Hb) icefishes display mean vessel length densities that are greater (Chaenocephalus aceratus, 5.51±0.32 mm mm-2; Champsocephalus gunnari, 5.15±0.50 mm mm-2) than those observed in red-blooded (+Hb) species (Gymnodraco acuticeps, 5.20±0.46 mm mm-2; Parachaenichthyes charcoti, 4.40±0.30 mm mm-2; Trematomus hansoni, 3.94±0.08 mm mm-2; Notothenia coriiceps, 2.48±0.21 mm mm-2). -Hb fishes also have mean vessel diameters that are -1.5 times greater than vessel diameters of +Hb species (-Hb, 0.193±0.006 mm; +Hb, 0.125±0.005 mm). Vascular density index (VDI), a stereological index that is affected by both vessel number and length, is greatest in -Hb C. aceratus (3.51±0.20) and lowest in +Hb N. coriiceps (1.58±0.14). Among four +Hb species, there is a direct relationship between red blood cell content and retinal vasculature. Hematocrit (Hct) is inversely correlated to vascular density (r2=0.934) and positively correlated to intervessel distance (r2= 0.898) over a >2.3-fold range of Hct. These results indicate that anatomical capacity to supply blood to the retina increases to compensate for decreases in oxygen-carrying capacity of the blood.