MICROCLONAL PROPAGATION OF CRATAEGUS MONOGYNA JACQ. IN VITRO

Abstract

We have researched Crataegus Monogina Jacq. microclonal propagation and in vitro mass regeneration processes. For this experiment we used the asleep buds isolated from maternal plants and in vitro cultivated apical and axillary buds as the effective explants. The high coefficient of micropropagation was provided with Gamborg Medium (B5) nutrient medium added alongside the hormones of cytokinin nature. The best result was achieved in the case of introduction of 2-Isopentenyladenine (2-iP) at concentration of 5-10 mcm and Benzilaminopurine (BAP) at concentration of 10-15 mcm. For stimulation of the mictropropagation process it is reasonable to add a small dose of the auxin hormone 1-Naphtylacetic acid (NAA) together with the cytokinins to the nutritive medium. It has been shown that the growth of micropropagation coefficient in pro rata the kinds and concentration of phytohormones. Regenerated microshoots had a good capacity of rooting. The most effective method for rooting purpose was the introduction of Indole- 3 –butyric acid (IBA) at concentration of 5mcm into 1 /2 B5 nutrient medium. The rooted regenerant plants successfully adopted in vivo.