Molecular identification of mango malformation pathogens in Egypt

Abstract

Diagnostic tests by molecular biology is made for studying the relations among Fusarium species for linking production of proteins, degree of relationship and occurrence of malformation. Determination of proteins for isolates causing-disease by SDS-PAGE explained there’s specific band for each fungus and there are common bands among some isolates of fungi. Since, band with MW 30 KDa represented only in F. proliferatum and F. oxysporum and F. subgluti- nans respectively. This band considered as specific band for these isolates, which released high patho- genisity effect. RAPD-PCR markers were used to discriminate variations between Fusarium isolates and causing disease. There is specific band for each fungus which act as molecular marker for each fun- gus and there are some bands common among some isolates of pathogenic fungi. The dendrogram shows there is degree of relationship between F. sterilihy- phosum and F. proliferatum; between F. moniliforme and F. subglutinans; between F. oxysporum and F. chlamydospore; the degree of relationship among F. subglutinans, F. proliferatum and F. sterilihyphosum and degree of relationship among F. moniliforme, F. sterilihyphosum, F. proliferatum and F. subglutinans.