Abstract
19randomamplifiedpolymorphicDNA(RAPD)andfiveInter-simplesequencerepeat(ISSR)primers wereusedtocharacterize35potatoaccessionsoriginatingfromMexico,EuropeandU.S.A,withthe aimofgeneratinginformationonthegeneticdiversityandrelationshipamongthegenotypesforbetter exploitationinbreedingprograms.Atotalof222and82lociweregeneratedbytheRAPDandISSR primers,respectively.BothRAPDandISSRprimersrevealed81.45and82.98%polymorphism, respectively.ManteltestshowednocorrelationbetweenRAPD andISSR (0.27),RAPD andRAPD +ISSR (0.47)similaritymatrices.However,ahighlevelofcorrelation(0.74)wasobservedbetweenISSRand RAPD+ISSRsimilaritymatrices.TheISSRmarkerwasfoundtobemoreefficientcomparedtoRAPD marker,therebyinfluencingmoretheconsensusdata.Theuseofconsensusdatageneratedmore informationrelatedtogeneticdiversityinpotato.RAPDandISSRmarkerssuccessfullydifferentiated betweenthepotatoaccessionsandsubgroupedthegenotypesbasedonorigin.Informationongenetic diversity andrelationshipswillbeusefulinthe selectionofparents andmappingstudies
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CITATION STYLE
Onamu, R., Legaria, J., Rodr iacute guez, J. L., Sahag ugrave n, J., & P egrave rez, J. (2016). Molecular characterization of potato (Solanum tuberosum L.) genotypes using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. African Journal of Biotechnology, 15(22), 1015–1025. https://doi.org/10.5897/ajb11.2656
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