UV-SPECTROPHOTOMETRIC ESTIMATION OF ACYCLOVIR IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Abstract

Anal. method development being a vital part of pre formulation-formulation research and development obviates the need to develop reliable, effective, eco friendly and cost effective methodologies for routine anal. of active pharmaceutical ingredients. UV spectroscopy is one of the earliest, yet of wide applications in drug anal. in different stages of formulations and quality control; despite the availabilities of sophisticated chromatog. techniques and other hyphenated techniques. Current research attempts to develop simple, sensitive, accurate, precise and economical UV spectrophotometric methods for the routine anal. of acyclovir in bulk and pharmaceutical dosage forms using two sep. alk. media, 0.1N NaOH (method A) and 0.1N KOH (method B) and validate them as per ICH guidelines. In both the methods max. absorbance was obsd. at 264 nm. Beer's law was obeyed in the concn. of 2.5-40 μg / mL in method A and 2.5-30 μg / mL in method B with correlation coeff. of 0.999. The % recovery carried out by adding known amt. of std. drug to pre-analyzed tablet solns. was 98.75 ± 0.52 % to 99.78 ± 0.69 % (method A) and 98.55 ± 0.31 % to 99.78 ± 0.22 % (method B). Intra and interday precision expressed in % RSD were 0.38 ± 0.01 and 0.27 ± 0.02 - 0.44 ± 0.01 resp. and the percent purity was 99.85 ± 0.05 %. The methods were validated statistically as per ICH guidelines and the results obtained were within the acceptance criteria for the parameters relating to linearity, accuracy, precision. [on SciFinder(R)]