The Genetic Diversity of Moringa Oleifera on Poteran Island-Madura Based on Petiole Colors Using ISSR ( Inter Simple Sequence Repeat ) Method

Abstract

Moringa oleifera, which is endemic of Pulau Poteran, Madura, is one of a genetic variation of Moringa. It is native Indonesian germplasm, which must be preserved to maintain the genetic biodiversity of Moringa. Known locally as kelor, the plant has four different color of petiole: red, white, purple, and green. The purpose of this study was to determine the genetic diversity of the plant with its four different colors of the petiole. This study used six ISSR molecular markers in the analysis. The stages of this research were the preparation of the leaf samples, the DNA extraction, the DNA quality and quantity observation, the PCR with ISSR (inter simple sequence repeat) primer, then the electrophoresis. The locus that shows bands was used for scoring: present (1) and is absent (0). Data analysis and the making of the dendrogram were done by using the unweighted pair group method arithmetic (UPGMA) via the MVSP program. The level of polymorphism generated by the ISSR marker was 97,2% with an average PIC of 0,46 and similarity value of 5%-66,7%.