The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

Wisnu Tafroji; Felicia Monica Bernadette; Ernawati A. Giri Rachman; Dodi Safari

Journal Article

The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

Journal of Microbiological Methods (2020) 172

DOI: 10.1016/j.mimet.2020.105909

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Abstract

We designed a sensitive and reliable method to distinguish serogroup 6 using PCR followed by enzymatic restriction digest. We discovered that this serotyping method was able to distinguish serotypes 6A, 6B, 6C, and 6D based on the recognition site of BsmAI in the wciP region.

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Tafroji, W., Bernadette, F. M., Giri Rachman, E. A., & Safari, D. (2020). The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae. Journal of Microbiological Methods, 172. https://doi.org/10.1016/j.mimet.2020.105909

The use of PCR and BsmAI restriction combination targeting wciP gene to determine serotype 6A, 6B, 6C and 6D Streptococcus pneumoniae

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