Nanolayered Double Hydroxide Inhibits the Pathogenicity of Vibrio parahaemolyticus

Abstract

Nanolayered double hydroxide (LDH) is a type of anion layered inorganic compound whose bacteriostatic properties have recently garnered much attention. Vibrio parahaemolyticus is a marine pathogen that can lead to aquaculture diseases and substantial economic losses. Therefore, our study assessed the mechanisms by which Mg/Al-LDH prevents V. parahaemolyticus infection. Our results demonstrated that Mg/Al-LDH not only inhibited V. parahaemolyticus growth but also biofilm formation. Moreover, coupling Mg/Al-LDH with hydrogen peroxide and UV irradiation further inhibited the growth and biofilm formation of V. parahaemolyticus. Additionally, Mg/Al-LDH was found to adversely affect DNA and the gelling ability of chitosan. Furthermore, exposing V. parahaemolyticus to Mg/Al-LDH led to a 54.73% and 4.3% inhibition in the expression of the toxic genes tlh and trh, respectively. Mg/Al-LDH also improved the symptoms of V. parahaemolyticus infection in Penaeus vannamei, making this a promising candidate to prevent pathogenic bacteria infection in aquaculture.