A molecular genetic evidence of the occurrence of the freshwater snail Radix lagotis (Schrank, 1803) (Gastropoda: Lymnaeidae) in Bulgaria

Abstract

Radix lagotis (Schrank, 1803) was found in the Dragoman marshes in Bulgaria. By comparison of the mitochondrial cyt-b sequences (fragment of 329 bp) and sequence data obtained from the nuclear ITS-2 spacer these specimens fell into one cluster with sequences from R. lagotis from Germany, Austria and Czech Republic confirming the morphological determination and the first record of this species for Bulgaria.