Exercise induced changes in T1, T2 relaxation times and blood flow in the lower extremities in healthy subjects

Abstract

Background: Current approaches to evaluate peripheral arterial disease (PAD) overwhelmingly rely on quantification of stenosis severity or flow across stenosis at rest. Although exercise-induced functional alterations in metabolism have been proposed, there is a need to integrate approaches that provide additional risk measures in PAD that can be translated into clinical practice [1]. Recently, quantitative T1/T2 mapping have allowed their usage as risk indicators in the coronary circulation. To develop analogous risk-markers in PAD, we initially designed experiments to assess the feasibility of an approach combining quantitative magnetic resonance imaging (MRI) relaxometry of skeletal muscle with treadmill exercise testing. We investigated exercise-induced shifts in quantitative skeletal muscle relaxometry measures [2,3] in lower extremity muscle beds in conjunction with quantitative arterial flow to the inflow vessel as an index of conduit vessel flow-reserve in healthy volunteers [4,5]. Methods: Fifteen subjects (5 each in age-based groups of 18-30, 30-50 and 50-70 yrs) exercised through Stage 3 Bruce protocol on an MRcompatible treadmill adjacent to the 1.5T MRI system. Axial T1 (TR/TE/ TI=1200/1.1/285 ms, FA=60°, 1 NEX, 1.4x1.4x8 mm3) and T2 (TR/TEs= 2500/ 0,24,26,48,60 ms, 3 NEX, 1.4x1.4x8 mm3 ) maps of the calf muscles, and blood flow in the superficial femoral artery (SFA) using cardiac triggered 2D cine phase contrast ( TR/TE=35.9/2.7 ms, 2 NEX, 40 heart phases, Venc =120 cm/s, 1x1x6 mm3) were obtained at rest, and every ∼5 minute intervals for ∼30 minutes after exercise. T1 and T2 changes in calf muscles (gastrocnemius, soleus and anterior tibialis) were measured in regions of (Figure presented) interest (ROI) on pre and post exercise maps (Figure 1), while blood flow was measured in the SFA supplying this muscle bed. Measured variables (averaged over both legs) were plotted over acquisition time points. Results: Blood flow and T1 exhibited a significant increase immediately post exercise followed by a progressive decline towards pre-exercise values. T2 only increased significantly in soleus muscle group (Figure 2). Conclusions: We have demonstrated the feasibility of assessing exercise induced changes in MRI relaxometric measures and compared these with changes in exercise induced flow in the lower extremities. The changes in T1 and T2 were generally coupled to changes in flow except in older age group some of whom had higher baseline T2 and abnormal post-exercise T2 recovery. We hypothesize that patients at risk for PAD related (Figure presented) complications may demonstrate abnormal kinetics of T1 and T2 recovery with exercise due to ischemia induced loss of microvascular integrity and tissue edema.