Studies on the propagation of Fritillaria camtschatcensis Ker-Gawl. by in vitro culture. I. Characteristics of sprouting, leafiness, flowering and new bulb formation from mother bulb, and bulblet formation from small globule-scale

Abstract

Wild black lily bulbs were planted in pots and grown for 8 months in a glasshouse. The bulbs produced scapes with 1 ~ 2 terminal flowers and 5 ~ 6 leaves with a verticillate phyllotaxis. Tow new bulbs were formed on the mother-bulb as it was consumed and eventually rotted. Morphological characteristics matched those of Fritillaria camtschatcensis Ker-Gawl. Small globule-scales of the bulbs were excised and cultured on MS-media without plant hormones in the dark or under light of 40 μmol · m-2 · sec-1 at 15, 20, and 25 °C for 8 weeks. The optimum condition for bulblet formation was a MS-medium containing a combination of 0.1 mg · liter-1 NAA and 0.1 mg · liter-1 24-epibrassinolide (EB) at 20 °C in the dark.