Abstract
We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.
Cite
CITATION STYLE
Charlebois, R. L., Lam, W. L., Cline, S. W., & Doolittle, W. F. (1987). Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium. Proceedings of the National Academy of Sciences of the United States of America, 84(23), 8530–8534. https://doi.org/10.1073/pnas.84.23.8530
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
