Standardization and Modification Techniques of Platelet-Rich Plasma (PRP) Preparation in Rabbit

Abstract

Background: Platelet-rich plasma (PRP) is used in several different fields of surgery to enhance natural healing by increasing concentrations of autologous platelets. However, there are controversial arguments in the literature considering the potential benefits of PRP, due to the deficiency of optimized and standardized preparation protocols. Aim: The aim of the present study is to standardize a method of autologous PRP preparation in rabbits, and also compare this methodology with our new modified method. Methods: 10 male New Zealand white rabbits were used in this study. After general anesthesia, 10ml of blood was collected from each animal via jugular vein, then the blood was divided into two equal amounts of 5ml. Blood samples were divided into two groups, and two different methods were implemented to prepare PRP. In group one, standardized technique with double centrifugation and in group two, our proposed modified protocol were used to prepare PRP with more than double centrifugation. Platelet count in PRP and whole blood samples with Neubauer slide. Results: The amount of platelet in PRP samples was almost four times greater than the platelet amount in peripheral blood samples. More precisely, the average whole blood platelet count was 496, 073±51,273/μl. The average of platelet in PRP samples in group one and two were 1,877,157±604,783/μl and 2,086,945±856,423/μl respectively, which were significantly higher than whole blood. Conclusion: It can be concluded that both of the methods used for producing autologous PRP show adequate platelet quantity and quality for rabbits.