Targeted Delivery of Doxorubicin in HeLa Cells Using Self-Assembled Polymeric Nanocarriers Guided by Deep Eutectic Solvents

Abstract

Green material/solvents have been extensively investigated for biomedical applications, including drug delivery applications.In this work, the material design of deep eutectic solvents (DESs) grafted chitosan (CS) with biotin nanocarrier was synthesized from green solvents or deep eutectic solvents and evaluated for sustained anticancer drug release of doxorubicin (DOX) drug. The chemical structure and physical properties of the DESs, DES-g-CS-Biotin, and DOX loaded polymeric nanocarrier were investigated via NMR, FT-IR, and density analysis. The surface morphology and size of the polymeric nanocarrier were determinedby Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM), and Dynamic Light Scattering (DLS) techniques. Furthermore, the sustained release of DOX loaded polymeric nanocarrier was studied using. UV-visible spectroscopy at the λmax value of 325 nm. The sustained release of drug discharge from the polymeric nanocarrier was evaluated under various physiological conditions.Moreover, the cell viability and cytotoxicity effects against polymeric nanocarrier and DOX loaded polymeric nanocarrier were evaluated using VERO and HeLa cell lines. In conclusion, the polymeric nanocarrier, with deep eutectic solvent enhances the bioavailability, and biocompatibility to biological system improves cancer chemotherapy treatment.