Effect of lifelong coenzyme Q10 supplementation on age-related oxidative stress and mitochondrial function in liver and skeletal muscle of rats fed on a polyunsaturated fatty acid (PUFA)-rich diet

Abstract

This study investigates aging-related changes in lipid peroxidation and functionality in liver and skeletal-muscle mitochondria in rats fed a diet rich in polyunsaturated fatty acids (PUFA), depending on supplementation or not with coenzyme Q10 (COQ10). Two groups of rats were fed for 24 months on a PUFA-rich diet, differing in supplementation or not with CoQ 10. At 6 and 24 months mitochondria were analyzed for fatty acid profile; hydroperoxides; α-tocopherol; CoQ9; CoQ10; cytochromes b, c+c1, and a+a3 contents; cytochrome c oxidase activity; and catalase activity in cytosol. Results of this study showed for the supplemented group an age-associated decrease in the peroxidizability index, an increase in catalase activity in skeletal muscle, and modulation of the aging-related changes in different mitochondrial electron-transport-chain components in skeletal muscle. These findings provide mechanisms to explain the effect of CoQ10 in extending the life span of animals fed a PUFA-rich diet. Copyright 2007 by The Gerontological Society of America.