Isolation and characterization of lupeol from the whole plant of Phaulopsis bateri

Abstract

This work was aimed at isolating and characterizing the bioactive constituents from the crude methanol extract of the whole plant of Phaulopsis barteri . The crude extract which was subjected to a silica gel column chromatography separation using petroleum ether/ethyl acetate blend, eluted a white crystalline substance with a melting point of 215-216 0 C. PBA shows recognizable peak at 79.02ppm which is characteristic of a carbon carrying an oxygen and the 1 HNMR spectrum which shows an olefinic proton at δ 4.71 and 4.56 respectively was identified as lupeol which is a C 30 H 50 O moiety. Identification of the compound was purely by spectral analysis. Keywords: Phaulopsis barteri , Lupeol, C 30 H 50 O, Chromatography