Characteristic of Orchid Mycorrhizal Fungi from Roots of Cypripedium japonicum and C. macranthum

Abstract

Department of Forestry, Chungbuk National University, Chengju 361-763, Korea(Received June 17, 2010. Accepted June 21, 2010)ABSTRACT: Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cyp-ripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the rootsof two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis.DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITSprimers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two speciesof Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.KEYWORDS : Cypripedium japonicum, Cypripedium macranthum, OMF, Orchid mycorrhizal fungi, Peloton, Tulasnellaceae