Identification des triacylglycérols en HPLC. Comment se passer du couplage HPLC-SM ? Dans quel cas la chromatographie est-elle encore indispensable ?

Abstract

Oils and fats are mainly composed of triacylglycerols. These differ in their total number of carbon atoms, the distribution of fatty acids on the three chains of the glycerol, and the configuration of fatty chains, linear, branched or cyclic. They also differ in the total number of double bonds, their position and configuration. This leads to complex mixtures with many congeners widely analyzed by conventional liquid chromatography. In this paper we show that whatever the detector (ELSD, Corona, MS) using only retention laws ln αvs. NC, ln αvs. NDL and ln αvs. NP allows us the identification of triacylglycerols in Equatorial Africa oils. The proposed approach does not require specific coupling with mass spectrometry. As example, we chose Ricinodendron heudelotii oil which mainly contains polyunsaturated positional isomers and configuration fatty acids C18:3.