Biomedical Evaluation of Biosynthesized Silver Nanoparticles by Jasminum Sambac (L.) Aiton Against Breast Cancer Cell Line, and Both Bacterial Strains Colonies

Abstract

The biosynthesis of silver nanoparticles (AgNPs) was conducted using the Iraqi Jasminum sambac (L.) Aiton leaves having substantial bioreduction and capping properties. The aqueous extract has been characterized using FTIR to observe changes in functional groups of extract compared to extract-AgNPs. GC-MS understands the mechanism synthesis of AgNPs based on the aqueous extract of J. sambac through identification of aqueous extracted. The synthesized AgNPs were characterized using UV-Vis at 455nm, XRD broad chart owing to size of AgNPs and TEM (AgNPs average size less than 10nm). FESEM-EDX was carried out to observe the nearly spherical shape with elemental composition. DLS was appointed with hydrodynamic radius as 105.9nm and also had a good polydispersity at 0.357, and Z-potential at -23.1. AgNPs have antibacterial gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli), cytotoxicity MTT assay against breast cancer MCF-7 cell line IC50 at 222.6μg/mL, genotoxicity fragmented DNA of MCF-7 by comet assay, emphasized apoptosis cells through cell cycle flow cytometry. Overall, safe, cost-effective, and scalable biogenic nano-formulation of Jasminum sambac-AgNPs possesses antibacterial and anticancer therapeutic applications.