Isolation and characterization of bacteria producing hydrolase enzymes from two moroccan hypersaline environments

Abstract

Halophilic bacteria are a group of microorganisms living in saline environments and in some cases need the salinity to survive. Furthermore,these bacteria species have the potential for interesting and promising applications. In fact, this is due not only to their ability to adapt to extreme physico-chemical conditions but also because many of them they produce interesting substances for the industry such as extracellular hydrolytic enzymes.The present study aims to isolate and characterize halophilic bacteria producing hydrolase enzymes from twosalinesin Sidi Moussa-Oualidia complex (Morocco) and to screen their potential to produce hydrolase enzymes. In this study, 15 halophilic bacteria were identified and analyzed for their ability to produce extracellular enzymes. The phylogenetic analysis based on 16S rDNA sequencing indicated that the 15 isolated strains belong to the genera Bacillus, Oceanobacillus and Virgibacillus. The study of enzymatic activity revealed that all isolates studied were capable of producing at least one extracellular hydrolytic enzyme of which 9 produced amylase, 6 cellulase, 13 DNase, 11 lipase, and 8 protease. In addition to their ability to produce extracellular hydrolytic enzymes, our isolates have demonstrated their potential adaption to extreme Physico-chemical conditions. These properties could allow them to be used for many industrial applications.