High performance liquid chromatography separation and analysis of metallotetra(pentafluoro-phenyl)porpholactone

Abstract

The analytical separation and analysis of silver, nickel, zinc and palladium complexes of tetra(pentafluorophenyl)porpho!actone (TFPL) from free bases is described. An isocratic mobile phase of n-hexane and diethyl ether was developed to separate these complexes using a normal phase pPorasil HPLC column (150 x 3.9 mm I. D.) at a flow rate of 1 mL/min. In addition, two porphodilactone isomers were also isolated from the reaction products, a result which we were unable to achieve with flash chromatography nor with a reversed phase C18 Ultrasphere HPLC column (250 x 4.6 mm I. D.). The optical absorption spectra of the metal derivatives show two bands in the visible region, compared to the four bands shown by the free base. The retention of these metal complexes on a normal phase column was found to be increasing in the order PdfTFPL) < AgfTFPL) < Zn(TFPL) < Ni(TFPL). Copyright © 1994 by Marcel Dekker, Inc.