High Pressure Carbon Dioxide Decreases the Heat Tolerance of the Bacterial Spores

Abstract

Effect of high pressure carbon dioxide treatment (35°C, 6.5 MPa) (HPCT) on the heat tolerance of bacterial spores was investigated. Spores of Bacillus coagulans and B. licheniformis were subjected to HPCT following heat treatment (80, 85 and 90°C for 30 min) (HT). Spores of both types were sterilized completely in 30 min-90°C HT after HPCT. These results indicated that HPCT greatly decreased the heat resistance of spores.