Bioactive oleanane glycosides from polyscias duplicata from the Madagascar dry forest [1]

Abstract

As part of the International Cooperative Biodiversity Group (ICBG) program, in a search for antiproliferative compounds, an ethanol extract of Polyscias duplicata was investigated due to its antiproliferative activity against the A2780 human ovarian cell cancer line (IC50 6 μg/mL). Seven known oleanane glycosides, 3β-[(α-L-arabinopyranosyl)oxy]-16α-hydroxyolean-12-en-28-oic acid (1, IC50 8 μM), 3β-[(α-L-arabinopyranosyl)oxy]-16α,23-dihydroxyolean-12-en-18-oic acid (2, IC50 13 μM), 3β-[(O-β-D-glucopyranosyl-(1.,-3)-α-L-arabinopyranosyl)oxy]-16α-hydroxyolean-12-en-28-oic acid (3, IC50 7 μM), 3β-[(O-α-L-rhamnopyranosyl-(1.,-2)-α-L-arabinopyranosyl)oxy]-16α-hydroxyolean-12-en-28-oic acid (4, IC50 2.8 μM), 3β-[(O-β-D-glucopyranosyl-(1.,-3)-α-Larabinopyranosyl) oxy]-23-hydroxyolean-12-en-28-oic acid (5, IC50 10 μM), 3β-[(O-α-L-rhamnopyranosyl-(1.,-2)-α-L-arabinopyranosyl)oxy]-23-hydroxyolean-12-en-28-oic acid (6, IC50 3.4 μM), and 3β-[(α-L-arabinopyranosyl)oxy]-23-hydroxyolean-12-en-28-oic acid (7, IC50 3.4 μM) were isolated, and their structures determined using spectroscopic methods.