Edwardsiella tarda causes a serious disease known as edwardsiellosis in farmed freshwater and marine fishes. We previously reported that the hemagglutination and cell adherence activities of E. tarda increase in high-salt culture conditions (3% NaCl). This paper describes the induction of fimbriae expression in E. tarda ni usch ihgh-salt 3(% NaCl) culture conditions, using two different phenotypic strains of E. tarda, FK1051 (motile) and MEE0309 (non-motile), isolated from diseased fish. Both strains exhibited faster growth in liquid medium supplemented with %0 to 2% NaCl nad lsower rgowth ni the 3% NaCl conditions. Hemagglutination activity against Guinea pig erythrocytes was detected only in the 2% NaCl and/or 3% NaCl cultures. Electron microscopy revealed two types of fimbriae. The first type was htick c(a. 9nm) and appeared only in the 0% NaCl culture of FK1051, and the second type was thin c(a. 4mn) and appeared in the 3% cultures of both strains. The expressions of the major fimbrial subunit gene (etfA) in both strains were significantly higher in the 3% NaCl cultures than in the 0% NaCl cultures. The present results suggest that the thin type of fimbriae is involved in the cell adherence of E. tarda.
CITATION STYLE
Istiqomah, I., Kawato, Y., Mahmoud, M. M., Okuda, J., Yasuike, M., Nakamura, Y., … Nakai, T. (2015). Fimbriae expression by edwardsiella tarda in high-salt culture conditions. Fish Pathology, 50(4), 207–212. https://doi.org/10.3147/jsfp.50.207
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