Production of Biogas from Artificial Substrates (oil, protein, and cellulose) by Indigenous Anaerob Bacteria

Abstract

Indigenous bacteria was isolated from POME, and the bacteria was applied to degrade oil, protein, and cellulose in an anaerobic condition and to produce biogas from artificial substrates, namely: oil, protein, and cellulose. Degradation process of the substrate was conducted in a bioreactor with degradation time of 3, 6, 9, 12 and 15 day. Stenotrophomonas rhizophila strain e-p10 as lipolytic bacteria dan Bacillus toyonensis strain BCT-711 as proteolytic and celulolytic bacteria were used in the degradation process. Specific growth rate, μ = 4.57 day-1 was obtained for lipolytic bacteria; 4.73 day-1 for proteolytic and 4.42 day-1 for cellulolytic bacteria. The optimum time for degradation of the substrate was 12 days. The production of methane achieves 47.04 % for lipolytic bacteria, 19.67% for proteolytic and 51.40% for cellulolytic bacteria.