The Genetic Diversity of Kemang ( Manifera kemaga Blume) collection from Bogor in the Germplasm Garden

Abstract

Kemang (Mangifera kemanga) is a wild relative of mango (Mangifera indica) with a tree-shaped habitus. Kemang was even designated as the identity flora of Bogor Regency. Several accessions of Kemang are found in the Cibinong Science Center Botanic Garden (CSC-BG) area, but their diversity has not been disclosed. This study aimed to provide information on the genetic diversity of the accessions Kemang in the CSC-BG Garden germplasm. A total of 17 out of 58 accessions were successfully obtained from young tissue samples, but for RAPD analysis, 13 accession numbers were used. The performance of each plant and physiological conditions and equipment became an obstacle to obtaining samples from all accessions. Further out of the 32 primers screened, 24 were selected (polymorphic), giving 312 clear and bright bands in 13 accessions. As much as 75.0% of primers were polymorphic, 6.25% monomorphic, and 18.75% did not produce bands. Furthermore, all primers had a moderate informative PIC value. The phylogenetic tree and scatter plot of PCoA show similar groupings. While the correlation between accession Kemang 2 and Kemang 13 has the highest closeness and vice versa in Kemang 7 and Kemang 6. These results provide information on the initial genetic diversity of the Kemang population in Germplasm Garden CSC BG.