Altered microtubule dynamics and vesicular transport in mouse and human MeCP2-deficient astrocytes

Abstract

Rett syndrome (RTT) is a rare X-linked neurodevelopmental disorder, characterized by normal post-natal development followed by a sudden deceleration in brain growth with progressive loss of acquired motor and language skills, stereotypic hand movements and severe cognitive impairment. Mutations in the methyl-CpG-binding protein 2 (MECP2) cause more than 95% of classic cases. Recently, it has been shown that the loss of Mecp2 from glia negatively influences neurons in a non-cellautonomous fashion, and that in Mecp2-null mice, re-expression of Mecp2 preferentially in astrocytes significantly improved locomotion and anxiety levels, restored respiratory abnormalities to a normal pattern and greatly prolonged lifespan compared with globally null mice. We now report that microtubule (MT)-dependent vesicle transport is altered in Mecp2-deficient astrocytes from newborn Mecp2-deficient mice compared with control wild-type littermates. Similar observation has been made in human MECP2 p. Arg294* iPSC-derived astrocytes. Importantly, administration of Epothilone D, a brain-penetrant MTstabilizing natural product, was found to restore MT dynamics in Mecp2-deficient astrocytes and in MECP2 p. Arg294* iPSCderived astrocytes in vitro. Finally, we report that relatively lowweekly doses of Epothilone Dalso partially reversed the impaired exploratory behavior in Mecp2308/y male mice. These findings represent a first step toward the validation of an innovative treatment for RTT.