Prevalence of Salmonella sp. Infections in layer flocks in East Azerbaijan province of Iran

Abstract

Salmonella is an important cause of human disease with an estimated 80.3 million annual foodborne cases and significant economical losses in the poultry sector worldwide. The aim of this study was to evaluation prevalence of Salmonella infections in layer flocks in East Azerbaijan province of Iran. For isolation of agent after autopsy of losses, liver surface was cauterized and swap from this area was obtained and added into the tetrathionate culture media and incubated at 37oC for 15 h Then agents obtained from previous stage were cultured in the macconkey agar culture media as five regional method and then incubated at 37oC. After several times, lightly yellow colonies appeared that suggestive non-fermentative lactose bacteria. For differentiation of Salmonella from other non-fermentative lactose bacteria, specific Medias such as TST, urea, MR-VP, SIM, simmon citrate, lysine and Medias contain sucrose, lactose, maltose, manoz and arabinose used. Finally, the amounts of losses since week 31 until 67 was 5030 hens that by adding losses before week 31 which was about 1450, the sum of losses was 6480 or 27% of flock. This study revealed that contamination rate in East Azerbaijan province of Iran is higher than standard levels and because of zoonotical issues between poultries and human must be take measures in this field. © Medwell lournals, 2012.