Multiple defence signals induced by Erwinia carotovora ssp. carotovora elicitors in potato

Abstract

Signal pathways involved in Solanum tuberosum-Erwinia carotovora ssp. carotovoraSCC3193 interaction were characterized. To this end, the concentration of several signal molecules implicated in plant defence such as ethylene (ET), jasmonates (JA) and salicylic acid (SA) were measured in potato plants treated by cell-free culture filtrates (CF) from E. c. carotovora SCC3193. Furthermore, the presence of other potential signalling compounds such as cinnamic acid (CA) and related aromatic compounds was screened in the elicitor-treated plants. The activity of these signal compounds as inducers of defence-related genes such as drd-1 (a defence-related alcohol dehydrogenase), pinII (proteinase inhibitor II), chtB4 (basic chitinase) and chtA2 (acidic chitinase) was characterized. The results demonstrate that ET, JA and CA accumulate in potato tissues in response to CF. These signal molecules were shown to induce differential expression of drd-1, pinII, chtB4 and chtA2. Our data suggest that in addition to ET and JA, CA and possibly other aromatic compounds also may play a role in defence signalling in potato. © 2005 Blackwell Publishing Ltd.