In vivo antioxidant and hepatoprotective potential of Glycyrrhiza glabra extract on carbon tetra chloride (CCl4) induced oxidative-stress mediated hepatotoxicity

Abstract

Objective: This study aimed to evaluate the antioxidant and hepatoprotective potential of Glycyrrhiza glabra hydromethanolic root extract against carbon tetra chloride (CCl4) induced oxidative-stress mediated hepatotoxicity in liver tissue of Swiss albino mice.Background: Medicinal plants play a vital role for the development of new drugs. Glycyrrhiza glabra is a widely used medicinal plant. It has many phyto-constituents and active components, which can be used for many diseases.Methods: For the antioxidant and hepatoprotective study, measurement of GSH, CAT, LPO bio-markers in Liver tissue of Swiss albino mice were taken. The animals were divided in six different groups each having 4 mice. The requisite dose of CCl4 was dissolved in appropriate solvent (1.5ml/kg body wt) and administrated as single i.p. dose per mice after 6 hr of last treatment of extract to the animals in each group. Mice were received orally administration of extract up to 7 days. Positive Control group received single i.p. injection of 1.5ml/kg body wt CCl4 in 0.9% saline.Results: The results suggest that, the crude extract of root of G. glabra at the doses of 300 and 600mg/kg body wt. expressed significant hepatoprotective potential against CCl4 induced oxidative stress mediated hepatotoxicity in student ‘t’ test (p<0.05) at dose dependent manner in the Liver tissue of Swiss albino mice. G. glabra root extract alone has not induced hepatotoxicity.Conclusion: Based on this study, It may be concluded that Glycyrrhiza glabra root extract possess hepatoprotective potential in Swiss albino mice.