Separation and quantification of the major daucane esters of Ferula hermonis by HPLC

Abstract

A simple HPLC method was developed to quantify the major daucane sesquiterpene esters present in herb and roots of Ferula hermonis Boiss. The method utilized a C18 reversed phase analytical column with isocratic elution for 30 minutes and UV detection at 240 nm. Extracts from two crude plant samples and two commercial products were fingerprinted and quantitatively analyzed.