Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform

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Abstract

A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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Leblanc, J. J., Pettipas, J., Davidson, R. J., Tipples, G. A., Hiebert, J., & Hatchette, T. F. (2008). Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform. Journal of Clinical Microbiology, 46(12), 4049–4051. https://doi.org/10.1128/JCM.01446-08

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