Synthetic and Medicinal Attributes of 1,2,4-Triazole Derivatives

Abstract

Background and aims: Skin aging is a life-annoying problem especially for women that needs to act so that appearance of signs of aging can be delayed as long as possible. Here, the current work was intended to discover the effects of eggshell membrane (EM), olive oil (OO), or castor oil (CO) as a skin anti-aging agent (SAAA). Materials and methods: The thirty-day study included the use of randomly-divided four groups of 20 rabbits, T1 (oral-administered with freshly egg-extracted EM 2gm/day), T2 (oral-administered with OO 5ml/day), T3 (oral-administered with CO 5ml/day), and T4 (nothing supplied). After the experiment was done, the parameters measured for detecting the effectiveness of the substances were component of skin amino acid (glycine, alanine, proline, and hydroxyproline). In addition, histopathological examination of the rabbit skin was conducted for 2 time points (one week and six weeks after starting of the experiment). Results: The results of T1 showed significant (p<0.01) higher percentages of components of amino acid in the rabbit skin than those from the other groups. Moreover, the histopathological examination revealed that T1 demonstrated the strongest proliferation of epidermal cells in the wrinkle of rabbit skin for the two time points when compared to the other groups. Conclusion: The current study demonstrated the promising effects of the eggshell membrane as a skin anti-aging agent.