Stability of thioamide type of piperine under acidic and basic conditions

Abstract

Thioamide type of piperine, “thiopiperine” is a derivate of piperine that having high potency against breast cancer cells. This research was intended to evaluate the stability of thiopiperine under highly acidic and basic conditions for 72 h at 60⁰C. This study was conducted by the SHIMADZU® UFLC system integrated with a PDA detector, while the analysis was performed in an isocratic separation mode using column C-18 (COSMOSIL®), 150 mm x 4.6 mm, column particle size: 5 µm. Chromatography condition was set using a mobile phase consisting of 50% aqueous acetonitrile with a flow rate of 1 mL/min, while the detection wavelength was 340 nm. The result showed that thiopiperine undergoes extensive degradation under acidic and basic environments.