Superoxide dismutase and catalase activities in the seminal plasma of normozoospermic and asthenozoospermic beagles

Abstract

We measured the blood plasma testosterone (T) levels and superoxide dismutase (SOD) and catalase activities in the seminal plasma of the ejaculates of 5 normal (2-5 years old) and 5 asthenozoospermic (AZ-) (3-5 years old) Beagles. Sperm ejaculated by AZ-dogs was incubated for 3 hr in Eagle's MEM only (controls) or Eagle's MEM containing 100 units/ml of SOD or catalase. Sperm motility was examined during incubation. The mean (± SE) plasma T level of the AZ-dogs (1.2 ± 0.2 ng/ml) was significantly lower than in the normal dogs (2.5 ± 0.2 ng/ml) (P<0.005). The mean (± SE) seminal plasma SOD and catalase activities (18.8 ± 1.9 and 0.5 ± 0.1 unit/g protein, respectively) were significantly lower in the AZ-dogs than in the normal dog (43.3 ± 2.5 and 2.2 ± 0.4 unit/g protein, respectively) (P<0.001 and 0.01, respectively). The motility of sperm incubated in Eagle's MEM containing SOD or catalase was significantly higher than that of control sperm incubated in only Eagle's MEM after 2 or 3 hr of incubation (P<0.05). The results of this study indicate that poor T secretion by the testes and low antioxidant enzyme activities are related to AZ in the dog.